PDE1C Assay Kit
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The PDE1C Assay Kit is designed for identification of PDE1C inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE1C to the binding agent. The key to the PDE1C Assay Kit is the_x000D_specific binding agent. Using this kit, only two simple steps on a microtiter plate are_x000D_required for PDE1C reactions. First, the fluorescently labeled cAMP is incubated with a_x000D_sample containing PDE1C for 1 hour. Second, a binding agent is added to the reaction_x000D_mix to produce a change in fluorescent polarization that can then be measured using a_x000D_fluorescence reader.
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