PDE8A Assay Kit

The PDE8A Assay Kit is designed for identification of PDE8A inhibitors_x000D_using fluorescence polarization. The assay is based on the binding of a fluorescent_x000D_nucleotide monophosphate generated by PDE8A to the binding agent. The key to the PDE8A_x000D_Assay Kit is the specific binding agent. Using this kit, only two simple steps on a_x000D_microtiter plate are required for PDE8A reactions. First, the fluorescently labeled cAMP_x000D_is incubated with a sample containing PDE8A for 1 hour. Second, a binding agent is_x000D_added to the reaction mix to produce a change in fluorescent polarization that can then_x000D_be measured using a fluorescence reader.