Caspase-1 Antibody

Caspase-1 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1β and IL-18. Caspase-1 was initially identified as an IL-1β-converting enzyme; later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Apoptosis, Cancer
• Host species: Rabbit
• Species reactivity: Human
• Homology: Predicted species reactivity based on immunogen sequence: Pig: (80%), Horse: (73%)
• Immunogen: Caspase-1 antibody was raised against a 15 amino acid synthetic peptide from near the middle of human Caspase-1.
• The immunogen is located within amino acids 200 - 250 of Caspase-1.
• Tested Applications: E, WB, IHC-P, IF
• Applications: Casp-1 antibody can be used for the detection of Caspase-1 by Western blot at 0.5 to 2 μ
• g/mL. Antibody can also be used for immunohistochemistry starting at 10 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in human samples
• Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: Depending on cell lines or tissues used, other cleavage products may be observed.
• Positive control 1: Cat. No. 1201 - HeLa Cell Lysate
• Positive control 2: Cat. No. 1306 - Human Spleen Tissue Lysate
• Positive control 3: Cat. No. 10-901 - Human Spleen Tissue Slide
• Positive control 4: Cat. No. 17-001 - HeLa Cell Slide
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: Caspase-1 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: Caspase-1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: CASP1
• Accession #: NP_150634
• Protein GI #: 15431328
• NCBI gene ID#: 834
• NCBI official full name: caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
• NCBI organism: Homo sapiens
• SWISSPROT #: P29466

Wysyłka i przechowywanie:

• Caspase-1 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.