Colon Membrane Lysate

Human colon tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human colon tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated colon tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated colon tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Specyfikacja/Zawartość:

• Lysate Type: Tissue
• Tested Applications: WB
• Applications: Colon membrane protein lysate is for use in Western blotting, 10 μ
• g to 20 μ
• g per lane is recommended for mini gel.
• Physical State: Liquid
• Buffer: HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
• Concentration: 2 mg/mL or better
• Source: Human Colon tissue
• Tissue by diagnosis: Normal
• Tissue by development stage: N/A
• Tissue by species: Human
• Tissue cell/type: Colon
• Diagnosis: Normal
• Cancer type: N/A
• Cancer extended: N/A
• Age: N/A
• Sex: N/A
• Race: N/A
• Species: Human
• Growth Media: N/A

Wysyłka i przechowywanie:

Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.

Inne:

This product is for research use only.