MCF7 Lysate

MCF7 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The MCF7 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Specyfikacja/Zawartość:

• Lysate Type: Cell Line
• Tested Applications: WB
• Applications: N/A
• Physical State: Liquid
• Buffer: MCF7 lysate is supplied in SDS sample buffer containing 50 mM DTT.
• Concentration: 2 mg/mL
• Source: N/A
• Tissue by diagnosis: Diseased
• Tissue by development stage: N/A
• Tissue by species: Human
• Tissue cell/type: MCF7 (Human Breast Adenocarcinoma)
• Diagnosis: Tumor
• Cancer type: N/A
• Cancer extended: N/A
• Age: N/A
• Sex: N/A
• Race: N/A
• Species: Human
• Growth Media: ATCC medium (Minimum essential medium Eagle with 2 mM L-glutamine and Earle’s BSS containing 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids and 1.0 mM sodium pyruvate, and 10% FBS)

Wysyłka i przechowywanie:

Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.

Inne:

This product is intended for laboratory research purposes only and should be used by qualified personnel only. ProSci lysates are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci lysates.