DEPDC1B Antibody

The DEP domain-containing 1B (DEPDC1B) protein was initially identified as a radiation response biomarker in human lymphoblastoid cell lines (1). Like the related protein DEPDC1A, expression of DEPDC1B correlates with a poor prognosis in cancer patients (2,3). Studies have shown that DEPDC1B levels are upregulated in non-small cell lung cancer (NSCLC) and have an inverse correlation with patient survival. Ectopic expression of DEPDC1B in NSCLC activated Wnt/B-catenin signaling and enhanced cell migration and invasion while silencing DEPDC1B expression suppressed these traits (2).

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Cancer, Cell Cycle
• Host species: Rabbit
• Species reactivity: Human
• Homology: Predicted species reactivity based on immunogen sequence: Mouse: (94%), Chicken: (88%)
• Immunogen: DEPDC1B antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DEPDC1B.
• The immunogen is located within the last 50 amino acids of DEPDC1B.
• Tested Applications: E, WB, IHC-P
• Applications: DEPDC1B antibody can be used for detection of DEPDC1B by Western blot at 1 - 2 μ
• g/ml. Antibody can also be used for immunohistochemistry starting at 5 μ
• g/mL.
• Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
• Specificity: DEPDC1B antibody is human specific. At least two isoforms are known to exist
• this antibody will only detect the largest isoform. DEPDC1B antibody is predicted to not cross-react with DEPDC1A.
• Positive control 1: Cat. No. 1204 - K562 Cell Lysate
• Positive control 2: Cat. No. 10-901 - Human Spleen Tissue Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 58 kDa
• Observed: 57 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: DEPDC1B antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: DEPDC1B antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: DEPDC1B
• Accession #: NP_060839
• Protein GI #: 223633999
• NCBI gene ID#: 55789
• NCBI official full name: DEP domain containing 1B
• NCBI organism: homo sapiens
• SWISSPROT #: Q8WUY9

Wysyłka i przechowywanie:

• DEPDC1B antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year.

Inne:

Optimal dilutions for each application to be determined by the researcher.