DIRAS3 Antibody

DIRAS3 is a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with mono-allelic expression of the paternal allele, which is associated with growth suppression and down-regulation of cyclin D1 promoter activity and induction of p21 (WAF/CIP1). Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers (1). DIRAS3 has been shown to induce autophagy in human ovarian cancer cells by blocking PI3K signaling, inhibiting the mammalian target of rapamycin (TOR), upregulating ATG4, and colocalizing with LC3 in autophagosomes (2). DIRAS also interacts with C-RAF and downregulates mitogen-activated protein kinase kinases (MEK) to restrict cell migration (3).

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Cell Cycle, Cancer, Autophagy
• Host species: Rabbit
• Species reactivity: Human
• Homology: N/A
• Immunogen: DIRAS3 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DIRAS3.
• The immunogen is located within the last 50 amino acids of DIRAS3.
• Tested Applications: E, WB
• Applications: DIRAS3 antibody can be used for detection of DIRAS3 by Western blot at 1 - 2 μ
• g/ml.
• Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
• Specificity: DIRAS3 antibody is human specific.
• Positive control 1: Cat. No. 1313 - Human Testis Tissue Lysate
• Positive control 2: N/A
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 25 kDa
• Observed: 24 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: DIRAS3 antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: DIRAS3 antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: DIRAS3
• Accession #: NP_004666
• Protein GI #: 4757772
• NCBI gene ID#: 9077
• NCBI official full name: DIRAS family, GTP-binding RAS-like 3
• NCBI organism: homo sapiens
• SWISSPROT #: O95661

Wysyłka i przechowywanie:

• DIRAS3 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year.

Inne:

Optimal dilutions for each application to be determined by the researcher.