JAK2 Antibody

JAK2 is a member of the JAK family of kinases that also include JAK1, JAK3, and TYK2 (1,2). JAK kinases are activated following tyrosine phosphorylation of cytokine receptors after ligand binding. JAK2 activation promotes the recruitment and phosphorylation of STAT3 and STAT5. These transcription factors then translocate to the nucleus where they bind specific DNA promoter sequences resulting in the transcription of genes that regulate cell proliferation, differentiation, and apoptosis (3). Mice with a disrupted JAK2 gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis (4).

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Stem Cell, Apoptosis
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: Predicted species reactivity based on immunogen sequence: Pig: (100%), Chicken: (82%)
• Immunogen: JAK2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human JAK2.
• The immunogen is located within the last 50 amino acids of JAK2.
• Tested Applications: E, WB, IHC-P
• Applications: JAK2 antibody can be used for detection of JAK2 by Western blot at 1 - 2 μ
• g/ml. Antibody can also be used for immunohistochemistry starting at 5 μ
• g/mL.
• Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
• Specificity: JAK2 antibody is human, mouse and rat reactive. JAK2 antibody is predicted to not cross-react with other members of the JAK family of proteins.
• Positive control 1: Cat. No. 1212 - 3T3 Cell Lysate
• Positive control 2: N/A
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 125 kDa
• Observed: 125 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: JAK2 antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: JAK2 antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: JAK2
• Accession #: NP_004963
• Protein GI #: 4826776
• NCBI gene ID#: 3717
• NCBI official full name: Janus kinase 2
• NCBI organism: homo sapiens
• SWISSPROT #: O60674

Wysyłka i przechowywanie:

• JAK2 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year.

Inne:

Optimal dilutions for each application to be determined by the researcher.