DAF Antibody

The decay-accelerating factor (DAF), also known as CD55, is an integral membrane glycoprotein that is involved in the regulation of the complement cascade (1). DAF limits excessive complement activation by accelerating their decay following DAF binding, thereby disrupting the cascade and preventing damage to host cells (2). Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM) (3).

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Immunology
• Host species: Rabbit
• Species reactivity: Human
• Homology: N/A
• Immunogen: DAF antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DAF.
• The immunogen is located within amino acids 360 - 410 of DAF.
• Tested Applications: E, WB, IHC-P, IF
• Applications: DAF body can be used for detection of DAF by Western blot at 1 - 2 μ
• g/ml. Antibody can also be used for Immunohistochemistry starting at 5 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in human samples
• Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: DAF antibody is human specific. At least two isoforms of DAF are known to exist
• this antibody will only detect the longest isoform.
• Positive control 1: Cat. No. 1203 - A549 Cell Lysate
• Positive control 2: Cat. No. 10-101 - Human Lung Tissue Slide
• Positive control 3: Cat. No. 1201 – HeLa Cell Lysate
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 48 kDa
• Observed: 49 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: DAF antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: DAF antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: CD55
• Accession #: NP_001108224
• Protein GI #: 168693643
• NCBI gene ID#: 1604
• NCBI official full name: CD55 molecule, decay accelerating factor for complement (Cromer blood group)
• NCBI organism: homo sapiens
• SWISSPROT #: P08174

Wysyłka i przechowywanie:

• DAF antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year.

Inne:

Optimal dilutions for each application to be determined by the researcher.