HDAC2 Antibody

The histone deacetylase (HDAC) family contains multiple members which are divided into four classes. Class I of the HDAC family comprises four members, HDAC1, 2, 3, and 8, each of which contains a deacetylase domain and exhibits a different, individual substrate specificity and function in vivo (1). HDAC2 was first identified as as a mammalian homolog to the yeast transcriptional regulator RPD3 (2). HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor (2,3). Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events (4).

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Cell Cycle, Stem Cell
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: Predicted species reactivity based on immunogen sequence: Chicken: (94%)
• Immunogen: HDAC2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human HDAC2.
• The immunogen is located within the last 50 amino acids of HDAC2.
• Tested Applications: E, WB, IHC-P, IF
• Applications: HDAC2 antibody can be used for detection of HDAC2 by Western blot at 0.5 - 1 μ
• g/ml. Antibody can also be used for Immunohistochemistry at 5 μ
• g/mL. For Immunoflorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in human samples
• Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
• Specificity: HDAC2 antibody is human, mouse and rat reactive. At least two isoforms of HDAC2 are known to exist
• this antibody will detect both isoforms. HDAC2 antibody is predicted to not cross-react with other members of the HDAC family.
• Positive control 1: Cat. No. 1201 - HeLa Cell Lysate
• Positive control 2: N/A
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 54 kDa
• Observed: 52 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: HDAC2 antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: HDAC2 antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: HDAC2
• Accession #: NP_001518
• Protein GI #: 293336691
• NCBI gene ID#: 3066
• NCBI official full name: histone deacetylase 2
• NCBI organism: homo sapiens
• SWISSPROT #: Q92769

Wysyłka i przechowywanie:

• HDAC2 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year.

Inne:

Optimal dilutions for each application to be determined by the researcher.