DOCK8 Antibody

The Dedicator of cytokinesis protein 8 (DOCK8) is a member of the DOCK180 family of guanine nucleotide exchange factors (1). DOCK8 plays an essential role in humoral immune responses and is important in the proper formation of the B cell immunological synapse (reviewed in 2). Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome (3).

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Immunology
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: N/A
• Immunogen: DOCK8 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human DOCK8.
• The immunogen is located within the last 50 amino acids of DOCK8.
• Tested Applications: E, WB, IHC-P
• Applications: DOCK8 antibody can be used for detection of DOCK8 by Western blot at 1 - 2 μ
• g/ml. Antibody can also be used for Immunohistochemistry at 5 μ
• g/mL.
• Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
• Specificity: DOCK8 antibody is human, mouse and rat reactive. Multiple isoforms of DOCK8 are known to exist.
• Positive control 1: Cat. No. 1287 - EL4 Cell Lysate
• Positive control 2: Cat. No. 10-901 - Human Spleen Tissue Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 166, 220, 231 kDa
• Observed: 220, 230 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: DOCK8 antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: DOCK8 antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: DOCK8
• Accession #: NP_982272
• Protein GI #: 238231392
• NCBI gene ID#: 81704
• NCBI official full name: dedicator of cytokinesis 8
• NCBI organism: homo sapiens
• SWISSPROT #: Q8NF50

Wysyłka i przechowywanie:

• DOCK8 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year.

Inne:

Optimal dilutions for each application to be determined by the researcher.