TET2 Antibody

TET2, a member of the ten-eleven-translocation (TET) family of genes, is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. It is a candidate tumor suppressor gene reported to be mutated in approximately 14% of patients with JAK2V617F-positive myeloproliferative neoplasms (1), and can be mutated in other hematopoietic disorders such as myelodysplastic syndromes, acute myeloid leukemia, and chronic myelomonocytic leukemia (2). Analysis of the TET2 and JAK2 mutations in these neoplasms suggests that mutations in TET2 do not represent a predisposition for acquiring mutations in JAK2.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Cancer, Cell Cycle
• Host species: Rabbit
• Species reactivity: Human, Mouse
• Homology: N/A
• Immunogen: TET2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human TET2.
• The immunogen is located within amino acids 1550 - 1600 of TET2.
• Tested Applications: E, WB
• Applications: TET2 antibody can be used for detection of TET2 by Western blot at 1 - 2 μ
• g/ml.
• Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
• Specificity: TET2 antibody is human and mouse reactive. At least two isoforms of TET2 are known to exist
• this antibody will detect the larger isoforms. This antibody is predicted to not cross-react with TET1 and TET3.
• Positive control 1: Cat. No. 1282 - 3T3 (NIH) Cell Lysate
• Positive control 2: N/A
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 220 kDa
• Observed: 220 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: TET2 antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: TET2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: TET2
• Accession #: NP_001120680
• Protein GI #: 187761317
• NCBI gene ID#: 54790
• NCBI official full name: tet methylcytosine dioxygenase 2
• NCBI organism: Homo sapiens
• SWISSPROT #: Q6N021

Wysyłka i przechowywanie:

• TET2 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year.

Inne:

Optimal dilutions for each application to be determined by the researcher.