MAP1LC3C Antibody

Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3C belongs to the MAP1 LC3 family of proteins that form mature complexes with MAP1A and MAP1B which are thought to be important in the formation and development of axons and dendrites (2). MAP1LC3C is one of three isoforms of MAP1LC3, the mammalian homolog of yeast ATG8, an essential autophagy protein. These isoforms exhibit distinct expression patterns and MAP1LC3C, like MAP1LC3A but not MAP1LC3B, is post-translationally modified, suggesting the three isoforms may have different physiological functions (3).

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Autophagy
• Host species: Rabbit
• Species reactivity: Human
• Homology: N/A
• Immunogen: MAP1LC3C antibody was raised against an 18 amino acid peptide near the carboxy terminus of human MAP1LC3C.
• The immunogen is located within the last 50 amino acids of MAP1LC3C.
• Tested Applications: E, WB, IHC-P, IF
• Applications: MAP1LC3C antibody can be used for detection of MAP1LC3C by Western blot at 1 - 2 μ
• g/ml. Antibody can also be used for Immunohistochemistry starting at 5 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in human samples
• Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: MAP1LC3C antibody is human, mouse and rat reactive. Multiple isoforms MAP1LC3C are known to exist. MAP1LC3C antibody is predicted to not cross-react with MAP1LC3A or MAP1LC3B.
• Positive control 1: Cat. No. 1303 - Human Brain Tissue Lysate
• Positive control 2: Cat. No. 10-301 - Human Brain Tissue Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 16 kDa
• Observed: 16 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: MAP1LC3C antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: MAP1LC3C antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: MAP1LC3C
• Accession #: NP_001004343
• Protein GI #: 51972260
• NCBI gene ID#: 440738
• NCBI official full name: microtubule-associated protein 1 light chain 3 gamma
• NCBI organism: homo sapiens
• SWISSPROT #: Q9BXW4

Wysyłka i przechowywanie:

• MAP1LC3C antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year.

Inne:

Optimal dilutions for each application to be determined by the researcher.