AIMP2 Antibody

AIMP2 was initially identified as a part of an aminoacyl-tRNA synthesase complex (1). It was later discovered to be a cofactor and substrate of Parkin, a Ring-type E3 ubiquitin ligase that is important for the survival of dopamine neurons in Parkinson’s disease; accumulation of AIMP2 in these cells lead to catecholaminergic cell death (2). AIMP2 can also bind to TRAF2, a key player in the TNF-alpha signaling pathway, causing the ubiquitination of TRAF2 by cIAP1, leading to TNF-alpha-dependent apoptosis (3). Finally, AIMP2 has been suggested to function as a tumor suppressor (4).

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Apoptosis
• Host species: Rabbit
• Species reactivity: Human
• Homology: N/A
• Immunogen: AIMP2 antibody was raised against a 16 amino acid peptide near the center of human AIMP2.
• The immunogen is located within amino acids 150 - 200 of AIMP2.
• Tested Applications: E, WB, IHC-P, IF
• Applications: AIMP2 antibody can be used for detection of AIMP2 by Western blot at 1 - 2 μ
• g/ml.
• Antibody validated: Western Blot in human samples
• Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
• Specificity: AIMP2 antibody is human specific. AIMP2 antibody is predicted to not cross-react with AIMP1.
• Positive control 1: Cat. No. 1201 - HeLa Cell Lysate
• Positive control 2: N/A
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 35 kDa
• Observed: 36 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: AIMP2 antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: AIMP2 antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: AIMP2
• Accession #: NP_006294
• Protein GI #: 11125770
• NCBI gene ID#: 7965
• NCBI official full name: aminoacyl tRNA synthetase complex-interacting multifunctional protein 2
• NCBI organism: homo sapiens
• SWISSPROT #: Q13155

Wysyłka i przechowywanie:

• AIMP2 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year.

Inne:

Optimal dilutions for each application to be determined by the researcher.