PHF14 Antibody

PHF14, a novel plant homology domain (PHD) transcription factor, controls the proliferation of mesenchymal cells by regulating PDGFR alpha expression in embryonic development (1). PHD finger transcription factors are concerned with transcriptional regulation by interacting with modified histones (2), and dysregulation of several genes encoding PHD finger transcription factors, such as ING1 and RBP2, has been found in human cancer diseases suggesting these factors have an important role for cancer cell proliferation. PHF14 is a potential target for new treatments of lung fibrosis (1, 3).

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Stem Cell
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: N/A
• Immunogen: PHF14 antibody was raised against a 19 amino acid peptide near the amino terminus of human PHF14.
• The immunogen is located within amino acids 210 - 260 of PHF14 .
• Tested Applications: E, WB, IF
• Applications: PHF14 antibody can be used for detection of PHF14 by Western blot at 1 - 2 μ
• g/ml.
• Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: PHF14 antibody is human, mouse and rat reactive. Multiple isoforms of PHF14 are known to exist.
• Positive control 1: Cat. No. 1210 - HEK293 Cell Lysate
• Positive control 2: Cat. No. 17-010 - HEK293 Cell Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 98 kDa
• Observed: 100kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: PHF14 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: PHF14 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: PHF14
• Accession #: NP_055475
• Protein GI #: 55769548
• NCBI gene ID#: 9678
• NCBI official full name: PHD finger protein 14
• NCBI organism: homo sapiens
• SWISSPROT #: O94880

Wysyłka i przechowywanie:

• PHF14 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year.

Inne:

Optimal dilutions for each application to be determined by the researcher.