ERAP1 Antibody

The endoplasmic reticulum (ER) aminopeptidase 1 (ERAP1), a member of the peptidase M1 family, plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides (1,2). It is also designated as adipocyte-derived leucine aminopeptidase (A-LAP), puromycin-insensitive leucine-specific aminopeptidase (PILS-AP) and aminopeptidase regulator of TNFR1 shedding (ARTS-1) (3). ERAP1 is localized to the lumen of the ER and induced by interferon. It may be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney (3,4).

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Innate Immunity
• Host species: Rabbit
• Species reactivity: Human, Mouse
• Homology: Predicted species reactivity based on immunogen sequence: Rat: (78%)
• Immunogen: ERAP1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ERAP.
• The immunogen is located within amino acids 730 - 780 of ERAP1 .
• Tested Applications: E, WB, IHC-P, IF
• Applications: ERAP1 antibody can be used for detection of ERAP1 by Western blot at 1 - 2 μ
• g/ml.
• Antibody validated: Western Blot in human samples
• Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Specificity: ERAP1 antibody is human and mouse reactive. At least two isoforms of ERAP1 are known to exist
• this antibody will detect both isoforms. ERAP1 antibody is predicted to not cross-react with ERAP2.
• Positive control 1: Cat. No. 1220 - SK-N-SH Cell Lysate
• Positive control 2: N/A
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 104 kDa
• Observed: 105kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: ERAP1 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: ERAP1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: ERAP1
• Accession #: NP_057526
• Protein GI #: 94818901
• NCBI gene ID#: 51752
• NCBI official full name: endoplasmic reticulum aminopeptidase 1
• NCBI organism: homo sapiens
• SWISSPROT #: Q9NZ08

Wysyłka i przechowywanie:

• ERAP1 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year.

Inne:

Optimal dilutions for each application to be determined by the researcher.