B-raf Antibody

B-raf Antibody: B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS), a disease characterized by heart defects, mental retardation and a distinctive facial appearance.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Cancer, Cell Cycle
• Host species: Rabbit
• Species reactivity: Human, Mouse
• Homology: Predicted species reactivity based on immunogen sequence: Rat: (100%), Chicken: (100%)
• Immunogen: Anti-B-raf antibody (7379) was raised against a peptide corresponding to 19 amino acids near the center of human B-raf.
• The immunogen is located within amino acids 330 - 380 of B-raf.
• Tested Applications: E, WB, IHC-P, IF
• Applications: WB: 0.5-2 μ
• g/mL
• IHC: 2.5 μ
• g/mL
• IF: 20 μ
• g/mL.
• Antibody validated: Western Blot in human and mouse samples
• Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: This antibody will not cross-react with C-raf.
• Positive control 1: Cat. No. 1303 - Human Brain Tissue Lysate
• Positive control 2: Cat. No. 11-801 - Human Small Intestine Tissue Slide
• Positive control 3: Cat. No. 1224 – Daudi Cell Lysate
• Positive control 4: Cat. No. 1204 – K562 Cell Lysate
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 94kD
• Observed: 94 kD
• MW explanation: N/A
• Validation:

  Independent Antibody Validation in Cell lines (Figure 2) shows similar B-raf expression profile in human and mouse cell lines detected by two independent anti-B-raf antibodies that recognize different epitopes, 5777 against central domain and 7379 against a slight different central domain. 

• B-raf proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 
• Isoforms: Human B-raf has one isoform (766aa, 84kD). Mouse B-raf has 2 isoforms, including isoform 1 (804aa, 89kD) and isoform 2 (329aa, 37kD). Rat B-raf a has one isoform (712aa, 80kD). 7379 can detect in human B-raf and mouse isoform 1.
• Purification: B-raf Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: B-raf Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: BRAF
• Accession #: P15056
• Protein GI #: 50403720
• NCBI gene ID#: 673
• NCBI official full name: v-raf murine sarcoma viral oncogene homolog B1
• NCBI organism: Homo sapiens
• SWISSPROT #: P15056

Wysyłka i przechowywanie:

• B-raf antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year.

Inne:

Optimal dilutions for each application to be determined by the researcher.