FCHO2 Antibody

FCHO2 Antibody: FCHO2 (FCH domain only 2) is a ubiquitously expressed member of the FCFBS superfamily characterized by FES-CIP4 homology (FCH) domain, an FBH domain, and an SH3 domain. Both FCHO2 and the related protein FCHO1 are mediators of clathrin-mediated endocytosis, acting to sculpt the initial bud site and recruit the clathrin machinery for clathrin-coated vesicle formation. FCHO2 binds to Eps15, an important adaptor protein in clathrin-mediated endocytosis.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Homeostasis
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: N/A
• Immunogen: FCHO2 antibody was raised against a 15 amino acid synthetic peptide near the center of human FCHO2.
• The immunogen is located within amino acids 340 - 390 of FCHO2.
• Tested Applications: E, WB, IHC-P, IF
• Applications: FCHO2 antibody can be used for detection of FCHO2 by Western blot at 1 - 2 μ
• g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ
• g/mL. For immunofluorescence start at 2.5 μ
• g/mL.
• Antibody validated: Western Blot in rat samples
• Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: FCHO2 antibody is predicted to not cross-react with other FCHO protein family members. At least the isoforms of FCHO2 are known to exist
• this antibody will detect only the two longest isoforms.
• Positive control 1: Cat. No. 1461 - Rat Heart Tissue Lysate
• Positive control 2: Cat. No. 10-501 - Human Heart Tissue Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: FCHO2 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: FCHO2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: FCHO2
• Accession #: NP_620137
• Protein GI #: 226371723
• NCBI gene ID#: 115548
• NCBI official full name: FCH domain only 2
• NCBI organism: Homo sapiens
• SWISSPROT #: Q0JRZ9

Wysyłka i przechowywanie:

• FCHO2 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.