SDHD Antibody

SDHD Antibody: The mitochondrial succinate dehydrogenase complex subunit D (SDHD) is one of four proteins that make up the tricarboxylic cycle enzyme succinate dehydrogenase (SCH). Studies have shown that mutations in SDHD often leads to hereditary paragangliomas, usually benign tumors of the autonomic nervous system, suggesting that SDHD also plays a role as a tumor-suppressor gene. In one family with a nonsense mutation (R22X) in the SDHD gene, a loss of heterozygosity was found in the paragangliomas, and within these tumors the enzymatic activity of Complex II in the mitochondrial respiratory chain was completely abolished. Furthermore, high levels of angiogenic factors EPAS1 and VEGF was observed, which may stimulate tumor growth.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Cancer, Cell Cycle
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%), Sheep: (100%), Pig: (80%)
• Immunogen: SDHD antibody was raised against a 15 amino acid synthetic peptide near the center of human SDHD.
• The immunogen is located within amino acids 30 - 80 of SDHD.
• Tested Applications: E, WB, ICC, IF
• Applications: SDHD antibody can be used for detection of SDHD by Western blot at 1 - 2 μ
• g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ
• g/mL. For immunofluorescence start at 2.5 μ
• g/mL.
• Antibody validated: Western Blot in mouse samples
• Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Specificity: At least four isoforms of SDHD are known to exist
• this antibody will detect the two longest isoforms.
• Positive control 1: Cat. No. 1287 - EL4 Cell Lysate
• Positive control 2: Cat. No. 17-207 - EL4 Cell Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: SDHD Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: SDHD Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: SDHD
• Accession #: NP_002993
• Protein GI #: 4506865
• NCBI gene ID#: 9392
• NCBI official full name: succinate dehydrogenase complex, subunit D, integral membrane protein
• NCBI organism: Homo sapiens
• SWISSPROT #: O14521

Wysyłka i przechowywanie:

• SDHD antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.