SHANK3 Antibody

SHANK3 Antibody: SH3 and multiple ankyrin repeat domains 3 (SHANK3), a member of the Shank gene family, plays a role in synapse formation and dendritic spine maturation. Shank proteins (Shank 1-3) containing PDZ domains are scaffold proteins of the postsynaptic density (PSD) that connect neurotransmitter receptors and ion channels proteins to the actin cytoskeleton and G-protein-coupled signaling pathways. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development. Mutations of SHANK3 are a cause of autism spectrum disorder (ASD) and the neurological symptoms of 22q13.3 deletion syndrome.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Neuroscience
• Host species: Rabbit
• Species reactivity: Human, Mouse
• Homology: N/A
• Immunogen: SHANK3 antibody was raised against a 19 amino acid synthetic peptide near the center of human SHANK3.
• The immunogen is located within amino acids 890 - 940 of SHANK3.
• Tested Applications: E, WB, IHC-P, IF
• Applications: SHANK3 antibody can be used for detection of SHANK3 by Western blot at 1 μ
• g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ
• g/mL. For immunofluorescence start at 2.5 μ
• g/mL.
• Antibody validated: Western Blot in mouse samples
• Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: At least three alternatively spliced transcript isoforms of SHANK3 are known to exist.
• Positive control 1: Cat. No. 1282 - 3T3 (NIH) Cell Lysate
• Positive control 2: Cat. No. 10-301 - Human Brain Tissue Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: SHANK3 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: SHANK3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: SHANK3
• Accession #: NP_001073889
• Protein GI #: 122937241
• NCBI gene ID#: 85358
• NCBI official full name: SH3 and multiple ankyrin repeat domains 3
• NCBI organism: Homo sapiens
• SWISSPROT #: Q9BYB0

Wysyłka i przechowywanie:

• SHANK3 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.