SUMO2/3 Antibody

SUMO2/3 Antibody: Small ubiquitin-like modifiers (SUMOs) are a family of small, related proteins (Sumo-1/2/3/4) that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation, which is a major regulator of protein function in cellular processes such as nuclear transport, transcriptional regulation, apoptosis and protein stability. All SUMO proteins localize to the nucleus and are covalently conjugated, affecting protein structure, function and interactions. SUMO2 and 3 are 96% identical and are more mobile within nucleus relative to SUMO1. Specific functional differences between SUMO1 and SUMO2 and 3 remain to be identified.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Homeostasis
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (93%), Pig: (93%)
• Immunogen: SUMO2/3 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human SUMO2.
• The immunogen is located within the last 50 amino acids of SUMO2/3.
• Tested Applications: E, WB, IHC-P, IF
• Applications: SUMO2/3 antibody can be used for detection of SUMO2 and SUMO3 by Western blot at 1 - 2 μ
• g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in human samples
• Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1205 - Jurkat Cell Lysate
• Positive control 2: N/A
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 15 kDa
• Observed: 15 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: SUMO2/3 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: SUMO2/3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: SUMO2
• Accession #: AAH71645
• Protein GI #: 47940079
• NCBI gene ID#: 6613
• NCBI official full name: SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
• NCBI organism: Homo sapiens
• SWISSPROT #: P61956

Wysyłka i przechowywanie:

• SUMO2/3 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.