APC2 Antibody

APC2 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC2 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. Like APC11, APC2 contains cullin and RING finger domains that are thought to be important in regulating ubiquitination activity.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Cancer, Cell Cycle
• Host species: Rabbit
• Species reactivity: Human, Mouse
• Homology: N/A
• Immunogen: APC2 antibody was raised against an 18 amino acid synthetic peptide near the center of human APC2.
• The immunogen is located within amino acids 240 - 290 of APC2.
• Tested Applications: E, WB, ICC, IF
• Applications: APC2 antibody can be used for detection of APC2 by Western blot at 1 - 2 μ
• g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in human samples
• Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1201 - HeLa Cell Lysate
• Positive control 2: Cat. No. 17-001 - HeLa Cell Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: APC2 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: APC2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: APC2
• Accession #: BAA34611
• Protein GI #: 3894265
• NCBI gene ID#: 10297
• NCBI official full name: adenomatosis polyposis coli 2
• NCBI organism: Homo sapiens
• SWISSPROT #: O95996

Wysyłka i przechowywanie:

• APC2 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.