PPAPDC1A Antibody

PPAPDC1A Antibody: Phosphatidate phosphatase (PAP) plays important role in lipid-signaling metabolism in eukaryotic cells. Two distinct types of PAP (PAP1 and PAP2) activity have been distinguished by their subcellular localization and differential sensitivity to N-ethylmaleimide(NEM) and Mg2+. A yeast diacylglycerol pyrophosphate (DGPP) phosphatase (DPP1) and mammalian DGPP phosphatase (PAP2) have been identified as Mg2+-independent and NEM-insensitive membrane-associated. PPAPDC1A (also known as DPPL2) and PPAPDC1B (DPPL1) form a novel type of Mg2+-independent and NEM-sensitive mammalian phosphatidate phosphatase showing broad substrate specificity. PPAPDC1A is preferentially expressed in endothelial cells. Studies of PPAPDC1A and PAP activity suggest that they may play a role in angiogenesis.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Signal Transduction
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: N/A
• Immunogen: PPAPDC1A antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human PPAPDC1A.
• The immunogen is located within the last 50 amino acids of PPAPDC1A.
• Tested Applications: E, WB, IF
• Applications: PPAPDC1A antibody can be used for detection of PPAPDC1A by Western blot at 1 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1303 - Human Brain Tissue Lysate
• Positive control 2: Cat. No. 10-301 - Human Brain Tissue Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: PPAPDC1A Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: PPAPDC1A Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: PPAPDC1A
• Accession #: NP_001025230
• Protein GI #: 73611920
• NCBI gene ID#: 196051
• NCBI official full name: phosphatidic acid phosphatase type 2 domain containing 1A
• NCBI organism: Homo sapiens
• SWISSPROT #: Q5VZY2

Wysyłka i przechowywanie:

• PPAPDC1A antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.