RIPK1 Antibody

RIPK1 Antibody: RIPK1 (Receptor Interacting Protein) is a crucial 74 kD adaptor kinase in several of stress-induced signaling pathways and on the crossroad of a cell's decision to live or die. RIPK1 contains an N-terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C-terminal region containing a death domain motif present in the Fas and TNFR1 intracellular domains. Full length RIPK1 is important for signallling to NFκ-B, MAPKs and necrosis, whereas caspase-8 generates a C-terminal RIPK1 cleavage fragment, promoting TNF-induced apoptosis. It is required for TNFRSF1A-mediated and TLR3-induced NF-κB activation. RIPK1-deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Apoptosis
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: N/A
• Immunogen: RIPK1 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human RIPK1.
• The immunogen is located within amino acids 180 - 230 of RIPK1.
• Tested Applications: E, WB, IHC-P, IF
• Applications: RIPK1 antibody can be used for detection of RIPK1 by Western blot at 1 μ
• g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in rat samples
• Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1465 - Rat Kidney Tissue Lysate
• Positive control 2: N/A
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: RIPK1 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: RIPK1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: RIPK1
• Accession #: NP_003795
• Protein GI #: 57242761
• NCBI gene ID#: 8737
• NCBI official full name: receptor (TNFRSF)-interacting serine-threonine kinase 1
• NCBI organism: Homo sapiens
• SWISSPROT #: Q13546

Wysyłka i przechowywanie:

• RIPK1 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.