Fibulin 3 Antibody

Fibulin 3 Antibody: Fibulin 3, also known as epidermal growth factor (EGF)-containing fibulin-like extracellular matrix protein 1 (EFEMP1), is a member of the fibulin family of extracellular glycoproteins, a group of proteins that are widely distributed and frequently associated with vascular and elastic tissues. The fibulin proteins typically contain a tandem array of EGF-like repeats and a fibulin-type COOH-terminal module. Aberrant accumulation of Fibulin 3 in the endoplasmic reticulum of retinal pigment epithelial cells has been shown to be associated with inherited forms of macular degeneration, but the loss of Fibulin 3 expression does not lead to macular degeneration but rather the appearance of hernias due to a reduction of elastic fibers of fascial connective tissue. Recent experiments have shown that expression of Fibulin 3 promotes tumor growth and may thus be a therapeutic target.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Cancer
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: N/A
• Immunogen: Fibulin 3 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human Fibulin 3.
• The immunogen is located within amino acids 410 - 460 of Fibulin 3.
• Tested Applications: E, WB, ICC, IF
• Applications: Fibulin 3 antibody can be used for detection of Fibulin 3 by Western blot at 0.5 - 1 μ
• g/mL. Antibody can also be used for immunocytochemistry starting at 20 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in human samples
• Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: At least three isoforms of Fibulin 3 are known to exist. This antibody is predicted to not cross-react with other Fibulin proteins.
• Positive control 1: Cat. No. 1201 - HeLa Cell Lysate
• Positive control 2: Cat. No. 17-001 - HeLa Cell Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 52 kDa
• Observed: 47 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: Fibulin 3 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: Fibulin 3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: EFEMP1
• Accession #: NP_001034437
• Protein GI #: 9665262
• NCBI gene ID#: 2202
• NCBI official full name: EGF-containing fibulin-like extracellular matrix protein 1
• NCBI organism: Homo sapiens
• SWISSPROT #: Q12805

Wysyłka i przechowywanie:

• Fibulin 3 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.