CDC42 Antibody

CDC42 Antibody: CDC42 is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to S. cerevisiae CDC42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. CDC42 is thought to regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. At least two isoforms of CDC42 are known to exist.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Homeostasis
• Host species: Chicken
• Species reactivity: Human, Mouse, Rat
• Homology: Predicted species reactivity based on immunogen sequence: Pig: (100%), Chicken: (100%), Bovine: (100%), Guinea pig: (100%)
• Immunogen: CDC42 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human CDC42.
• The immunogen is located within amino acids 40 - 90 of CDC42.
• Tested Applications: E, WB
• Applications: CDC42 antibody can be used for detection of CDC42 by Western blot at 0.5 - 1 μ
• g/mL.
• Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1303 - Human Brain Tissue Lysate
• Positive control 2: N/A
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: CDC42 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgY
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: CDC42 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: CDC42
• Accession #: NP_001782
• Protein GI #: 4757952
• NCBI gene ID#: 998
• NCBI official full name: cell division cycle 42 (GTP binding protein, 25kDa)
• NCBI organism: Homo sapiens
• SWISSPROT #: P60953

Wysyłka i przechowywanie:

• CDC42 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.