RCAN2 Antibody

RCAN2 Antibody: Regulator of calcineurin 2 (RCAN2), also known as ZAKI4 and DSCR1L1, is expressed as two isoforms differing at their N-terminus. The longer of the two (isoform 1) is expressed exclusively in the brain, while isoform 2 is ubiquitously expressed, with highest expression in brain, heart, and muscle. Both isoforms bind to the catalytic subunit of calcineurin, a Ca++-dependent protein phosphatase involved in several neuronal functions, though their C-terminal region and inhibit calcineurin's activity. Unlike isoform 1 of RCAN2, the expression of the second isoform is not induced by the thyroid hormone T3. RCAN2 is a member of a family of three endogenous calcineurin regulators that are located near the minimal supernumerary fragment of chromosome 21 in individuals with Down syndrome, suggesting that they play a role in this syndrome. Multiple isoforms of RCAN2 are known to exist.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Neuroscience
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)
• Immunogen: RCAN2 antibody was raised against a 14 amino acid synthetic peptide near the center of human RCAN2.
• The immunogen is located within amino acids 70 - 120 of RCAN2.
• Tested Applications: E, WB, IHC-P, IF
• Applications: RCAN2 antibody can be used for detection of RCAN2 by Western blot at 1 - 2 μ
• g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in mouse samples
• Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1282 - 3T3 (NIH) Cell Lysate
• Positive control 2: N/A
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: RCAN2 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: RCAN2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: Rcan2
• Accession #: AAH62141
• Protein GI #: 38328420
• NCBI gene ID#: 53901
• NCBI official full name: regulator of calcineurin 2
• NCBI organism: Mus musculus
• SWISSPROT #: Q9JHG2

Wysyłka i przechowywanie:

• RCAN2 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.