EBI3 Antibody

EBI3 Antibody: EBI3 is a subunit in two distinct heterodimeric cytokines: interleukin-27 (IL-27) and IL-35. Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 (also known as TCCR) and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. IL-35 is composed of EBI3 and the p35 subunit of IL-12 and has been reported to have therapeutic effects against collagen-induced arthritis by expanding the population of regulatory T cells and suppressing Th17 cells. At least two isoform of EBI3 are known to exist.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Immunology
• Host species: Rabbit
• Species reactivity: Human
• Homology: Predicted species reactivity based on immunogen sequence: Mouse: (46.7%), Rat: (46.7%)
• Immunogen: Anti-EBI-3 antibody (4967) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of mature human EBI-3.
• The immunogen is located within the last 50 amino acids of EBI3.
• Tested Applications: E, WB, IHC-P, IF
• Applications: WB: 1-4 μ
• g/mL
• IF: 20 μ
• g/mL
• IHC: 2.5 μ
• g/mL
• .
• Antibody validated: Western Blot, Immunofluorescence, and Immunohistochemistry in human samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1315 - Human Tonsil Tissue Lysate
• Positive control 2: Cat. No. 10-901 - Human Spleen Tissue Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 25kD
• Observed: 30 kD (25kD + 2 N-Glycosylations)
• MW explanation: N/A
• Validation:

  Recombinant Protein Test (Figure 2) shows that anti-EBI-3 antibody (4967) detects human recombinant IL-17 (IL-17A + IL-17B) protein that contains the amino acids of 4967 antigenic peptide.

   

• Isoforms: Human EBI-3 has only 1 isoform (229aa, 25kD). Mouse EBI-3 has one isoform (228aa, 25kD) and rat EBI-3 also has one isoform (228aa, 25kD). 4967 can detect human EBI-3 but cannot detect mouse and rat EBI-3.
• Purification: EBI3 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: EBI3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: EBI3
• Accession #: NP_005746
• Protein GI #: 14577917
• NCBI gene ID#: 10148
• NCBI official full name: Epstein-Barr virus induced 3
• NCBI organism: Homo sapiens
• SWISSPROT #: Q14213

Wysyłka i przechowywanie:

• EBI3 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.