CDCA8 Antibody

CDCA8 Antibody: CDCA8 is a component of a chromosomal passenger complex (CPC) required for stability of the bipolar mitotic spindle. The chromosomal passenger complex, which includes Survivin, CDCA8, INCENP and Aurora-B, is known to play crucial roles during mitosis and cell division. It was found that CDCA8 interacting with Aurora-B, INCENP and Survivin, increases during G2/M phase and then reduces after exit from M phase. CDCA8 is cell cycle regulated, down-regulated in response to p53/Rb-signaling, and up-regulated in many types of cancerous tissues. In Drosophila cells, inactivation of CDCA8 results in polyploidy, delayed mitosis and abnormal tissue development, indicating its critical role for cell proliferation. Recent studies show that CDCA8 is essential for cell proliferation during early embryonic development, and its early embryonic lethality cannot be rescued by the loss of p53. Its aberrant expression is linked to a poor prognosis for gastric cancer.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Stem Cell
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: N/A
• Immunogen: CDCA8 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of the human CDCA8.
• The immunogen is located within the first 50 amino acids of CDCA8.
• Tested Applications: E, WB, IF
• Applications: CDCA8 antibody can be used for detection of CDCA8 by Western blot at 1 and 2 μ
• g/mL. Antibody can also be used for immunoflourescence starting at 20 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1465 - Rat Kidney Tissue Lysate
• Positive control 2: N/A
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: CDCA8 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: CDCA8 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: CDCA8
• Accession #: NP_060571
• Protein GI #: 8922438
• NCBI gene ID#: 55143
• NCBI official full name: cell division cycle associated 8
• NCBI organism: Homo sapiens
• SWISSPROT #: Q53HL2

Wysyłka i przechowywanie:

• CDCA8 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.