CAPS2 Antibody

CAPS2 Antibody: CAPS2 and the related protein CAPS1 encode novel neural/endocrine-specific cytosolic and peripheral membrane proteins. Both are essential components of the synaptic vesicle priming machinery and are required for the Ca2+-regulated exocytosis of secretory vesicles; CAPS-deficient neurons contain no or very few fusion competent synaptic vesicles, causing a selective impairment of fast phasic transmitter release. Like CAPS1, numerous isoforms of CAPS2 are known to exist. These isoforms show distinct expression patterns in the brain. For example, CAPS2b showed high expression in the developing cerebellum. Furthermore, one version of CAPS2 mRNA that lacks exon 3 is detected in some autistic patients, suggesting that the differential expression pattern of CAPS2 is involved in neuronal development.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Neuroscience
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: N/A
• Immunogen: CAPS2 antibody was raised against a 19 amino acid synthetic peptide near the center of the human CAPS2.
• The immunogen is located within amino acids 510 - 560 of CAPS2.
• Tested Applications: E, WB, IHC-P, IF
• Applications: CAPS2 antibody can be used for detection of CAPS2 by Western blot at 0.5 - 1 μ
• g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in human samples
• Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: Multiple isoforms of CAPS2 are known to exist. This CAPS2 antibody is predicted to be specific to CAPS2 and not recognize CAPS1.
• Positive control 1: Cat. No. 1303 - Human Brain Tissue Lysate
• Positive control 2: Cat. No. 10-301 - Human Brain Tissue Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 138, 145 kDa
• Observed: 150 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: CAPS2 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: CAPS2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: CADPS2
• Accession #: NP_060424
• Protein GI #: 148839294
• NCBI gene ID#: 93664
• NCBI official full name: Ca++-dependent secretion activator 2
• NCBI organism: Homo sapiens
• SWISSPROT #: Q86UW7

Wysyłka i przechowywanie:

• CAPS2 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.