RAP80 Antibody

RAP80 Antibody: RAP80 was initially identified as zinc-finger containing nuclear protein that is highly expressed in testis and interacts with the retinoid-related testis-associated receptor (RTR). Later experiments revealed that RAP80 is recruited by the Coiled-coil domain 98 (CCDC98) protein to the breast cancer-1 protein BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both RAP80 and CCDC98 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either RAP80 or CCDC98 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that RAP80 and CCDC98 control only part of the DNA damage response role of BRCA1. At least four isoforms of RAP80 are known to exist.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Homeostasis
• Host species: Rabbit
• Species reactivity: Human, Mouse
• Homology: Predicted species reactivity based on immunogen sequence: Pig: (81%)
• Immunogen: RAP80 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human RAP80.
• The immunogen is located within amino acids 520 - 570 of RAP80.
• Tested Applications: E, WB, IHC-P, IF
• Applications: RAP80 antibody can be used for detection of RAP80 by Western blot at 2 μ
• g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in human samples
• Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1210 - HEK293 Cell Lysate
• Positive control 2: Cat. No. 10-901 - Human Spleen Tissue Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: RAP80 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: RAP80 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: UIMC1
• Accession #: EAW85043
• Protein GI #: 119605449
• NCBI gene ID#: 51720
• NCBI official full name: ubiquitin interaction motif containing 1
• NCBI organism: Homo sapiens
• SWISSPROT #: Q96RL1

Wysyłka i przechowywanie:

• RAP80 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.