STEAP1 Antibody

STEAP1 Antibody: The six-transmembrane epithelial antigen of prostate 1 (STEAP1) was the first member of a family of metalloreductases identified as cell-surface antigens in prostate tissue. The normal function of STEAP is still uncertain; unlike other members of the STEAP family, STEAP1 does not promote iron or copper reduction or uptake and lacks the FNO-like reductase domain critical for activity. However, its expression is highly increased in multiple cancer cell lines, including prostate, bladder, colon, and ovarian cancers. Supporting this is evidence that STEAP1 peptides can be used to stimulate CD8+ T cells from healthy donors, enabling them to recognize STEAP1-positive human tumor cells, suggesting that STEAP1 may a potential target for cancer immunotherapy. At least three isoforms of STEAP1 are known to exist.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Homeostasis, Cancer
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: Predicted species reactivity based on immunogen sequence: Pig: (94%)
• Immunogen: STEAP1 antibody was raised against a 16 amino acid synthetic peptide from near the center of human STEAP1.
• The immunogen is located within amino acids 130 - 180 of STEAP1.
• Tested Applications: E, WB, IHC-P, IF
• Applications: STEAP1 antibody can be used for detection of STEAP1 by Western blot at 1 - 2 μ
• g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL. better
• Antibody validated: Western Blot in human samples
• Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: This STEAP1 antibody does not cross-react with other STEAP proteins.
• Positive control 1: Cat. No. 1306 - Human Spleen Tissue Lysate
• Positive control 2: Cat. No. 10-901 - Human Spleen Tissue Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: STEAP1 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: STEAP1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: STEAP1
• Accession #: EAL24166
• Protein GI #: 51094921
• NCBI gene ID#: 26872
• NCBI official full name: six transmembrane epithelial antigen of the prostate 1
• NCBI organism: Homo sapiens
• SWISSPROT #: Q9UHE8

Wysyłka i przechowywanie:

• STEAP1 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.