DISC1 Antibody

DISC1 Antibody: Disrupted in schizophrenia 1 (DISC1) is a candidate gene for susceptibility to schizophrenia. It was discovered through chromosomal analysis of a large Scottish family whose members exhibited schizophrenia and related psychiatric disorders. Through yeast two-hybrid screening, it was discovered that DISC1 interacts with many members of the centrosome and cytoskeletal system including MAP1A and Nudel. More recently, DISC1 has been found to regulate the transport of a complex containing Nudel, the lissencephaly-1 (LIS1) protein, and 14-3-3epsilon from neuronal cell bodies to the axons by the action of the microtubule-dependent directed motor protein kinesin-1, also known as KIF5A. Decreased expression of DISC1 in neurons caused an accelerated rate of neuronal integration, resulting in aberrant morphological development, suggesting that DISC1 plays a role in dendritic development and synapse formation.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Neuroscience
• Host species: Rabbit
• Species reactivity: Human, Mouse
• Homology: Predicted species reactivity based on immunogen sequence: Rat: (88%)
• Immunogen: DISC1 antibody was raised against an 18 amino acid synthetic peptide from near the center of human DISC1.
• The immunogen is located within amino acids 350 - 400 of DISC1.
• Tested Applications: E, WB, ICC, IF
• Applications: DISC1 antibody can be used for detection of DISC1 by Western blot at 0.5 - 2 μ
• g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in human samples
• Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: At least four isoforms of DISC1 are known to exist
• this antibody will detect the three longest isoforms.
• Positive control 1: Cat. No. 1220 - SK-N-SH Cell Lysate
• Positive control 2: Cat. No. 17-001 - HeLa Cell Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 72, 92, 94 kDa
• Observed: 94 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: DISC1 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: DISC1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: DISC1
• Accession #: NP_061132
• Protein GI #: 61742823
• NCBI gene ID#: 27185
• NCBI official full name: disrupted in schizophrenia 1
• NCBI organism: Homo sapiens
• SWISSPROT #: Q9NRI5

Wysyłka i przechowywanie:

• DISC1 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.