Bub1 Antibody

Bub1 Antibody: Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Homeostasis, Signal Transduction, Cancer
• Host species: Rabbit
• Species reactivity: Human, Mouse
• Homology: N/A
• Immunogen: Bub1 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human Bub1.
• The immunogen is located within amino acids 40 - 90 of Bub1.
• Tested Applications: E, WB, ICC, IF
• Applications: Bub1 antibody can be used for detection of bub1 by Western blot at 2 - 4 μ
• g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in mouse samples
• Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1288 - A20 Cell Lysate
• Positive control 2: Cat. No. 17-208 - A-20 Cell Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: Bub1 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: Bub1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: BUB1
• Accession #: O43683
• Protein GI #: 8134347
• NCBI gene ID#: 699
• NCBI official full name: budding uninhibited by benzimidazoles 1 homolog (yeast)
• NCBI organism: Homo sapiens
• SWISSPROT #: O43683

Wysyłka i przechowywanie:

• Bub1 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.