MDA5 Antibody

MDA5 Antibody: The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-κB. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Innate Immunity
• Host species: Rabbit
• Species reactivity: Human, Mouse
• Homology: N/A
• Immunogen: MDA5 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human MDA5.
• The immunogen is located within the last 50 amino acids of MDA5.
• Tested Applications: E, WB, IHC-P, IF
• Applications: MDA5 antibody can be used for detection of MDA5 by Western blot at 1 - 4 μ
• g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in human samples
• Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1224 - Daudi Cell Lysate
• Positive control 2: Cat. No. 1369 - Human Lymph node Tissue Lysate
• Positive control 3: Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 113 kDa
• Observed: 115 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: MDA5 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: MDA5 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: IFIH1
• Accession #: NP_071451
• Protein GI #: 27886568
• NCBI gene ID#: 64135
• NCBI official full name: interferon induced with helicase C domain 1
• NCBI organism: Homo sapiens
• SWISSPROT #: Q9BYX4

Wysyłka i przechowywanie:

• MDA5 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.