TSLP Antibody

TSLP Antibody: Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. TSLP is a four-helix-bundle cytokine that is expressed mainly by barrier epithelial cells and is a potent activator of several cell types such as myeloid dendritic cells. TSLP is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Amino acid sequence analysis has shown poor homology between human and mouse TSLP although they exhibit similar biological functions and are expressed in similar tissues. At least two differentially spliced isoforms of TSLP are known to exist.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Immunology
• Host species: Rabbit
• Species reactivity: Human
• Homology: N/A
• Immunogen: Anti-TSLP antibody (4021) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human TSLP.
• The immunogen is located within the last 50 amino acids of TSLP.
• Tested Applications: E, WB, IHC-P
• Applications: WB: 0.25 - 2 μ
• g/mL
• IHC-P: 2.5 μ
• g/mL.
• Antibody validated: Western Blot in human samples
• Immunohistochemistry and Immunocytochemistry in human samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1205 - Jurkat Cell Lysate
• Positive control 2: Cat. No. 1303 - Human Brain Tissue Lysate
• Positive control 3: Cat. No. 10-301 - Human Brain Tissue Slide
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 18 kD
• Observed: 26 kD ( Post-modifications: 2 N-linked glycosylations).
• MW explanation: N/A
• Validation:

  Recombinant Protein Validation (Figure 1): Anit-TSLP antibodies (4021) detected human TSLP recombinant protein at different concentrations.

  Regulated Expression Validation (Figure 4-8): Anit-TSLP antibodies (4021) detected increased expression in multiple tissue or cells after treatment with cytokines or chemicals, in which the increase in the expression was inhibited by co-treatment with anti-cholinergic drugs (Figure 5).

• Isoforms: Human TSLP has 2 isoforms, including isoform 1 (159aa, 18kD) and isoform 2 (63aa, 7kD). Mouse TSLP has one isoform (140aa, 16kD) and rat TSLP also has one isoform (135aa, 16kD). 4021 can detect only human isoforms, but cannot detect mouse and rat TSLP.
• Purification: TSLP Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: TSLP Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: TSLP
• Accession #: NP_149024
• Protein GI #: 14719428
• NCBI gene ID#: 85480
• NCBI official full name: thymic stromal lymphopoietin
• NCBI organism: Homo sapiens
• SWISSPROT #: Q969D9

Wysyłka i przechowywanie:

• TSLP antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.