Cbl Antibody

Cbl Antibody: The mammalian cbl family of ubiquitin ligases consists of three homologs known as cbl (also known as c-Cbl), Cbl-B, and Cbl-3 which share highly conserved a tyrosine-kinase-binding domain, linker and RING finger domain in their amino-terminal halves. Similar to other E3 ubiquitin ligases, Cbl catalyzes the transfer of ubiquitin from an E2 or Ubc (ubiquitin-conjugating) enzyme to the e-amino group of a lysine residue of the substrate protein. Cbl acts to negatively regulate many types of cell-surface receptors, including the Syk protein tyrosine kinase family. Cbl is thought to be involved in T- and B-cell signaling, in addition to thymus development. Of the three known homologs in the cbl family, cbl antibody reacts specifically with cbl. Multiple isoforms of cbl have been reported.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Signal Transduction, Cancer
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: N/A
• Immunogen: Cbl antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human cbl.
• The immunogen is located within amino acids 810 - 860 of Cbl.
• Tested Applications: E, WB, IF
• Applications: Cbl antibody can be used for detection of cbl by Western blot at 0.5 - 2 μ
• g/mL. Antibody can also be used for immunoflourescence starting at 20 μ
• g/mL.
• Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1224 - Daudi Cell Lysate
• Positive control 2: Cat. No. 1369 - Human Lymph node Tissue Lysate
• Positive control 3: Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: Cbl Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: Cbl Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: CBL
• Accession #: P22681
• Protein GI #: 115855
• NCBI gene ID#: 867
• NCBI official full name: Cas-Br-M (murine) ecotropic retroviral transforming sequence
• NCBI organism: Homo sapiens
• SWISSPROT #: P22681

Wysyłka i przechowywanie:

• Cbl antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.