Slug Antibody

Slug Antibody: Slug, a member of the Snail family of C2H2-type zinc finger transcription factors, was initially identified to be involved in epithelial-mesenchymal transitions as well as the formation of the neural tube during vertebrate embryogenesis. Like Snail, Slug transcription can be induced by growth factors such as FGF, BMP, and TGF-beta. Once expressed, Slug will bind E-box regions of promoters and repress transcription of genes such as E-cadherin and Claudin-1. More recently, its expression in breast, esophogeal, and colorectal carcinomas has been correlated with poor prognosis for survival. Furthermore, Slug can protect hemapoietic progenitor cells from radiation-induced apoptosis by repressing the p53-mediated transcription of Puma, a BH3-only antagonist of the anti-apoptotic members of the Bcl-2 family. Slug antibody has no cross-reactivity to Snail protein.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Signal Transduction, Cancer
• Host species: Rabbit
• Species reactivity: Human, Mouse
• Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%), Rat: (100%)
• Immunogen: Slug antibody was raised against a 14 amino acid synthetic peptide from near the center of human Slug.
• The immunogen is located within amino acids 70 - 120 of Slug.
• Tested Applications: E, WB
• Applications: Slug antibody can be used for detection of Slug by Western blot at 1 to 2 μ
• g/mL.
• Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1305 - Human Kidney Tissue Lysate
• Positive control 2: N/A
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: Slug Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: Slug Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: SNAI2
• Accession #: O43623
• Protein GI #: 11134406
• NCBI gene ID#: 6591
• NCBI official full name: snail homolog 2 (Drosophila)
• NCBI organism: Homo sapiens
• SWISSPROT #: O43623

Wysyłka i przechowywanie:

• Slug antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.