PKR Antibody

PKR Antibody: The interferon-inducible, double-stranded RNA (dsRNA)-dependent protein kinase PKR is a member of the eukaryotic initiation factor-2 alpha (eIF2-alpha) kinase family, possessing serine-threonine kinase activity and two dsRNA-binding motifs that acts as part of the innate immune system. Upon binding dsRNA, PKR undergoes a conformational change leading to its activation and its phosphorylation of the translation factor eIF2, resulting in a general shutdown of protein synthesis and induction of apoptosis through upregulation of caspase-8 and capsase-9 activity in order to prevent the production of more viruses. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Innate Immunity
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: N/A
• Immunogen: Anti-PKR antibody (3947) was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human PKR.
• The immunogen is located within the last 50 amino acids of PKR.
• Tested Applications: E, WB, IHC-P, IF
• Applications: WB: 1 μ
• g/mL
• IF: 20 μg/mL
• IHC-P: 2.5-5 μg/mL.
• Antibody validated: Western Blot in human and mouse samples
• Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Specificity: At least two different isoforms of PKR are known to exist
• this antibody will detect both isoforms.
• Positive control 1: Cat. No. 1202 - A431 Cell Lysate
• Positive control 2: Cat. No. 1462 - Rat Lung Tissue Lysate
• Positive control 3: Cat. No. 1208 - THP-1 cell lysate
• Positive control 4: Cat. No. 1224 - Daudi Cell Lysate
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 62kD
• Observed: 68kD
• MW explanation: N/A
• Validation:

  Independent Antibody Validation in Cell lines (Figure 2) shows similar PKR expression profile in human and mouse cell lines detected by two independent anti-PKR antibodies that recognize different epitopes, 3947 against C-terminus domain and 3949 against N-terminus domain.  

• PKR proteins are detected in all the tested human cell lines except SK-N-SH cell line at different expression levels  
• by the two independent antibodies.  
• Isoforms: Human PKR has 2 isoforms, including isoform 1 (551aa, 62.1kD) and isoform S (510aa, 57.4kD. This antibody detects both human isoforms. Mouse PKR has 1 isoform (515aa, 58.3kD). Rat PKR has only one isoform identified so far (513aa, 58.3kD).
• Purification: PKR Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: PKR Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: EIF2AK2
• Accession #: AAP57628
• Protein GI #: 31747519
• NCBI gene ID#: 5610
• NCBI official full name: eukaryotic translation initiation factor 2-alpha kinase 2
• NCBI organism: Homo sapiens
• SWISSPROT #: P19525

Wysyłka i przechowywanie:

• PKR antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.