TRPC3 Antibody

TRPC3 Antibody: The mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the "canonical TRP" (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to be components of receptor-regulated cation channels in different cell types. Furthermore, the members of the TRPC3/6/7 subfamily can be activated by diacylglycerol analogs, suggesting a possible mechanism of activation of these channels by PLC-coupled receptors. TRPC3 encodes a Ca2+-permeant channel that is agonist-activated but not store-operated or directly receptor-activated. TRPC3 physically interacts with TRPC6 and TRPC7 and forms functional tetrameric channels.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Signal Transduction, Neuroscience, Infectious Disease
• Host species: Rabbit
• Species reactivity: Human
• Homology: Predicted species reactivity based on immunogen sequence: Rat: (100%)
• Immunogen: TRPC3 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human TRPC3.
• The immunogen is located within amino acids 120 - 170 of TRPC3.
• Tested Applications: E, WB, IHC-P
• Applications: TRPC3 antibody can be used for the detection of TRPC3 by Western blot at 1 - 2 μ
• g/mL. Antibody can also be used for immunohistochemistry starting at 10 μ
• g/mL.
• Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
• Specificity: This antibody may cross-react with TRPC6 and TRPC7.
• Positive control 1: Cat. No. 1301 - Human Heart Tissue Lysate
• Positive control 2: N/A
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 93 kDa
• Observed: 100 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: TRPC3 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: TRPC3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: TRPC3
• Accession #: CAA74083
• Protein GI #: 2225937
• NCBI gene ID#: 7222
• NCBI official full name: transient receptor potential cation channel, subfamily C, member 3
• NCBI organism: Homo sapiens
• SWISSPROT #: Q13507

Wysyłka i przechowywanie:

• TRPC3 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.