TWEAK Antibody

TWEAK Antibody: TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane. TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis, induction of inflammatory cytokines, in addition to stimulation of apoptosis. The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Apoptosis, Cancer
• Host species: Rabbit
• Species reactivity: Human, Mouse
• Homology: N/A
• Immunogen: Anti-TWEAK antibody (3791) was raised against recombinant human TWEAK protein.
• Tested Applications: E, WB, IHC-P, IF
• Applications: WB: 0.5-4 μ
• g/mL
• IHC: 5-10 μ
• g/mL
• IF: 20 μ
• g/mL.
• Antibody validated: Western Blot in human and mouse samples
• Immunohistochemistry in human and mouse samples
• Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1303 - Human Brain Tissue Lysate
• Positive control 2: Cat. No. 1201 - HeLa Cell Lysate
• Positive control 3: Cat. No. 10-301 - Human Brain Tissue Slide
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 27 and 36 kD
• Observed: 27 and 36 kD
• MW explanation: N/A
• Validation:

  Recombinant Protein Test (Figure 2): Anti-TWEAK antibodies (3791) detected human TWEAK recombinant protein at different concentrations.

• Isoforms: Human TWEAK has 2 isoforms, including isoform 1 (249aa, 27kD) and isoform 2 (330aa, 36kD). Mouse TWEAK has one isoform (249aa, 27kD) and Rat TWEAK also has one isoform (249aa, 27kD).
• Purification: TWEAK Antibody is Protein A-purified.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: TWEAK Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: TNFSF12
• Accession #: NP_003800
• Protein GI #: 4507597
• NCBI gene ID#: 8742
• NCBI official full name: tumor necrosis factor (ligand) superfamily, member 12
• NCBI organism: Homo sapiens
• SWISSPROT #: O43508

Wysyłka i przechowywanie:

• TWEAK antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.