GITRL Antibody

GITRL Antibody: The tumor necrosis factor (TNF) and TNF receptor (TNFR) gene superfamilies regulate numerous biological functions including cell proliferation, differentiation, and survival through regulating the activation of the transcription factor NF-κB and various mitogen-activated protein kinases. The glucocorticoid-induced tumor necrosis factor receptor (GITR) is an emerging member of this family that is expressed on CD4+ CD25+ regulatory T cells and appears to have crucial immune regulation functions. Its ligand GITRL is expressed in endothelial and antigen-presenting cells and can activate NF-κB, induce both pro- and anti-apoptotic effects, inhibit the suppressive activity of regulatory T cells, and co-stimulate responder T cells through GITR. Dominant negative forms of NIK and TRAF2 expressed in transfected 293 cells substantially inhibited NF-κB activation, suggesting that the GITRL-GITR pathway involves both NIK and TRAF2.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Chemokines & Cytokines
• Host species: Rabbit
• Species reactivity: Human, Mouse
• Homology: N/A
• Immunogen: GITRL antibody was raised against purified recombinant human GITR ligand.
• Tested Applications: E, WB, ICC
• Applications: GITRL antibody can be used for the detection of GITRL by Western blot at 1 μ
• g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ
• g/mL.
• Antibody validated: Immunocytochemistry in human samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1208 - THP-1 Cell Lysate
• Positive control 2: Cat. No. 17-008 - THP-1 Cell Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: GITRL Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: GITRL Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: TNFSF18
• Accession #: Q9UNG2
• Protein GI #: 13124621
• NCBI gene ID#: 8995
• NCBI official full name: tumor necrosis factor (ligand) superfamily, member 18
• NCBI organism: Homo sapiens
• SWISSPROT #: Q9UNG2

Wysyłka i przechowywanie:

• GITRL antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.