TSC2 Antibody

TSC2 Antibody: Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 complex decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing that overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. At least three isoforms of TSC2 exist.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Signal Transduction
• Host species: Rabbit
• Species reactivity: Human, Mouse
• Homology: Predicted species reactivity based on immunogen sequence: Rat: (86%)
• Immunogen: TSC2 (NT) was raised against a 14 amino acid synthetic peptide from near the amino terminus of human TSC2.
• The immunogen is located within the first 50 amino acids of TSC2.
• Tested Applications: E, WB, ICC, IF
• Applications: TSC2 antibody can be used for the detection of TSC2 by Western blot at 2 and 4 μ
• g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in mouse samples
• Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1284 - L1210 Cell Lysate
• Positive control 2: Cat. No. 17-204 - L1210 Cell Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: TSC2 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: TSC2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: TSC2
• Accession #: NP_000539
• Protein GI #: 116256352
• NCBI gene ID#: 7249
• NCBI official full name: tuberous sclerosis 2
• NCBI organism: Homo sapiens
• SWISSPROT #: P49815

Wysyłka i przechowywanie:

• TSC2 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.