XAF-1 Antibody

XAF-1 Antibody: XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death. XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines. Both IFN-alpha2 and IFN-beta can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Apoptosis
• Host species: Rabbit
• Species reactivity: Human, Mouse
• Homology: N/A
• Immunogen: XAF-1 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human XAF-1.
• The immunogen is located within the last 50 amino acids of XAF-1.
• Tested Applications: E, WB, IHC-P, IF
• Applications: XAF-1 antibody can be used for the detection of XAF-1 by Western blot at 0.5 to 2 μ
• g/mL. Antibody can also be used for immunohistochemistry starting at 2 μ
• g/mL. For immunofluorescence start at 10 μ
• g/mL.
• Antibody validated: Western Blot in human samples
• Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1306 - Human Spleen Tissue Lysate
• Positive control 2: Cat. No. 10-901 - Human Spleen Tissue Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 35 kDa
• Observed: 32 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: XAF-1 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: XAF-1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: XAF1
• Accession #: CAA68030
• Protein GI #: 1869901
• NCBI gene ID#: 54739
• NCBI official full name: XIAP associated factor 1
• NCBI organism: Homo sapiens
• SWISSPROT #: Q6GPH4

Wysyłka i przechowywanie:

• XAF-1 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.