KLHL1 Antibody

KLHL1 Antibody: The mammalian Kelch-like 1 (KLHL1) was initially discovered as a homolog to the Drosophila Kelch gene that is highly expressed in several brain tissues. The predicted protein domain structure of KLHL1 is characteristic of a number of proteins that bind actin, form dimers, and often act as actin-organizing proteins. Based on the presence of anti-sense RNA that spans the transcription and translation start sites as well as the first splice site of KLHL1 in brain tissue of individuals suffering from the neurodegenerative disorder spinocerebellar ataxia type 8 (SCA8), it has been suggested that KLHL1 is involved this disease and that regulation of KLHL1 protein may be affected by antisense RNA expression.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Neuroscience
• Host species: Rabbit
• Species reactivity: Human, Rat
• Homology: Predicted species reactivity based on immunogen sequence: Mouse: (100%)
• Immunogen: KLHL1 antibody was raised against a 14 amino acid synthetic peptide from near the amino-terminus of human KLHL1.
• The immunogen is located within the first 50 amino acids of KLHL1.
• Tested Applications: E, WB, IHC-P
• Applications: KLHL1 antibody can be used for the detection of KLHL1 by Western blot at 2 - 4 μ
• g/mL. Antibody can also be used for immunohistochemistry starting at 10 μ
• g/mL.
• Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1201 - HeLa Cell Lysate
• Positive control 2: Cat. No. 1403 - Mouse Brain Tissue Lysate
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 82 kDa
• Observed: 82 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: KLHL1 Antibody is immunoaffinity purified .
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: KLHL1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: KLHL1
• Accession #: AAF81719
• Protein GI #: 8926179
• NCBI gene ID#: 57626
• NCBI official full name: kelch-like 1 (Drosophila)
• NCBI organism: Homo sapiens
• SWISSPROT #: Q9NR64

Wysyłka i przechowywanie:

• KLHL1 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.