CIDE-B Antibody

CIDE-B Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICAD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-B induces apoptosis, which is inhibited by DFF45. CIDE-B is a DFF45-inhibitable effector that promotes cell death and DNA fragmentation. CIDE-B is expressed mainly in liver and at lower levels in spleen, kidney, peripheral blood lymphocytes and bone marrow.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Apoptosis
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: Predicted species reactivity based on immunogen sequence: Bovine: (86%)
• Immunogen: CIDE-B antibody was raised against a peptide corresponding to 14 amino acids near the center of human CIDE-B.
• The immunogen is located within amino acids 100 - 150 of CIDE-B.
• Tested Applications: E, WB, IHC-P, IF
• Applications: CIDE-B antibody can be used for detection of CIDE-B by Western blot at 0.5 - 2 μ
• g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in mouse samples
• Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: CIDE-B antibody has no cross activity to CIDE-A.
• Positive control 1: Cat. No. 1408 - Mouse Small Intestine Tissue Lysate
• Positive control 2: Cat. No. 1308 - Human Small Intestine Tissue Lysate
• Positive control 3: Cat. No. 11-801 - Human Small Intestine Tissue Slide
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: CIDE-B Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: CIDE-B Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• NCBI official symbol: CIDEB
• Accession #: AF218586
• Protein GI #: 6746626
• NCBI gene ID#: 27141
• NCBI official full name: cell death-inducing DFFA-like effector b
• NCBI organism: Homo sapiens
• SWISSPROT #: Q9UHD4

Wysyłka i przechowywanie:

• CIDE-B antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.