DFF40 Antibody

DFF40 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively. DFF45/ICAD is the inhibitory protein of DFF40/CAD and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Apoptosis
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: Predicted species reactivity based on immunogen sequence: Bovine: (94%)
• Immunogen: DFF40 antibody was raised against a peptide corresponding to 18 amino acids near the center of murine CAD.
• The immunogen is located within amino acids 130 - 180 of DFF40.
• Tested Applications: E, WB, ICC, IF
• Applications: DFF40 antibody can be used for detection of DFF40 by Western blot 0.5 μ
• g/mL. A 40 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in human samples
• Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1204 - K562 Cell Lysate
• Positive control 2: Cat. No. 1205 - Jurkat Cell Lysate
• Positive control 3: Cat. No. 17-005 - Jurkat Cell Slide
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 38 kDa
• Observed: 40 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: DFF40 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: DFF40 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: Dffb
• Accession #: NP_031885
• Protein GI #: 160948620
• NCBI gene ID#: 13368
• NCBI official full name: DNA fragmentation factor, beta subunit
• NCBI organism: Mus musculus
• SWISSPROT #: O54788

Wysyłka i przechowywanie:

• DFF40 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.