Tim-3 Recombinant Protein

The TIM (T cell/transmembrane, immunoglobulin and mucin) family plays a critical role in regulating immune responses, including allergy, asthma, transplant tolerance, autoimmunity and the response to viral infections. The unique structure of TIM immunoglobulin variable region domains allows highly specific recognition of phosphatidylserine (PtdSer), exposed on the surface of apoptotic cells. Tim-3, a type I transmembrane protein, contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion. TIM-3 is preferentially expressed on Th1 and Tc1 cells, and generates an inhibitory signal resulting in apoptosis of Th1 and Tc1 cells. TIM-3 is also expressed on some dendritic cells and can mediate phagocytosis of apoptotic cells and cross-presentation of antigen. Tim-3 functions to inhibit aggressive Th1-mediated auto- and alloimmune responses. Tim-3 pathway blockade by administration of Tim-3:Fc fusion protein accelerates diabetes in nonobese diabetic mice, causes hyperproliferation of Th1 cells and Th1 cytokine release in an experimental autoimmune encephalomyelitis (EAE) model and prevents acquisition of transplantation tolerance induced by costimulation blockade.

Specyfikacja/Zawartość:

• Tested Applications: N/A
• Applications: This recombinant proteins is for research use only.
• Predicted Molecular Weight: N/A
• Physical state: Lyophilized
• Buffer: Lyophilized from 0.2um-filtered solution in PBS. Reconstitute with 100 µl sterile water. Add 1X PBS to the desired protein concentration.
• Concentration: N/A
• NCBI official symbol: HAVCR2
• Accession #: NP_116171
• Protein GI: 49574534
• NCBI gene ID#: 84868
• NCBI official full name: hepatitis A virus cellular receptor 2 
• NCBI organism: Homo sapiens
• Peptide sequence: N/A
• SWISSPROT #: O77457
• Background Reference 1: N/A
• Background Reference 2: N/A
• Background Reference 3: N/A
• Background Reference 4: N/A
• Background Reference 5: N/A
• Source: CHO cells
• Species: Human
• By Source: CHO Cells
• By Species: Human
• Fusion tag: Fc Tag
• Sequence: The extracellular domain of human Tim-3 (aa 22-200) is fused to the N-terminus of the Fc region of mouse IgG2a.
• Biology activity: Measured by its binding ability in a functional ELISA.
• Purity: >98% (SDS-PAGE).
• Endotoxin level is less than 0.06EU/ µg protein (LAL test
• Lonza).

Wysyłka i przechowywanie:

Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Inne:

Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.