Nectin-2 Recombinant Protein

Poliovirus receptor-related 2 (PVRL2) is also known as nectin-2 and CD112 (formerly herpesvirus entry mediator B, HVEB), which is a human plasma membrane glycoprotein. PVRL2 is one of the plasma membrane components of adherens junctions. PVRL2 also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in PVRL2 gene have been associated with differences in the severity of multiple sclerosis. Also, PVRL2 binds with low affinity to TIGIT. Furthermore, PVRL2 acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV).

Specyfikacja/Zawartość:

• Tested Applications: E, WB
• Applications: This recombinant protein can be used for E, WB. For research use only.
• Predicted Molecular Weight: 37.4 kDa
• Physical state: Lyophilized
• Buffer: PBS, pH7.4
• Concentration: N/A
• NCBI official symbol: Nectin-2
• Accession #: AAH03091
• Protein GI: N/A
• NCBI gene ID#: 5819
• NCBI official full name: Poliovirus receptor-related 2 (herpesvirus entry mediator B)
• NCBI organism: Homo sapiens
• Peptide sequence: Gln 32 - Leu 360
• SWISSPROT #: NP_002847.1
• Background Reference 1: Martinez W.M., et al., 2001, J. Virol. 75:11185-11195.
• Background Reference 2: Yu X., et al., 2009, Nat. Immunol. 10:48-57.
• Background Reference 3: N/A
• Background Reference 4: N/A
• Background Reference 5: N/A
• Source: HEK293 cells
• Species: Human
• By Source: Human Cells
• By Species: Human
• Fusion tag: His Tag
• Sequence: Gln 32 - Leu 360
• Biology activity: Measured by its binding ability in a functional ELISA. Immobilized Human TIGIT, Fc Tag at 10 ug/mL (100 ul/well),can bind Human Nectin-2, His Tag with a linear range of 0.16-5 ug/mL (Routinely tested).
• Purity: >95% as determined by SDS-PAGE.
• Endotoxin level is less than 1.0 EU per ug by the LAL method.

Wysyłka i przechowywanie:

Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

Inne:

Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.