CD40L multimeric Recombinant Protein

MultimericCD40L™ is a high activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to [CD40L+enhancer] combinations. MultimericCD40L™ has shown to suppress alum-induced IL-1beta release and caspase-1 activation in a dose-, CD40- and time dependent manner, without affecting BMDM viability. It also effectively suppressed the inflammasome function triggered by NLRP3 activators. The secretion of caspase-1 independent inflammatory mediators has been shown to be unaltered or even enhanced.

Specyfikacja/Zawartość:

• Tested Applications: N/A
• Applications: This recombinant proteins is for research use only.
• Predicted Molecular Weight: N/A
• Physical state: Lyophilized
• Buffer: Contains PBS Reconstitute with 100 µl sterile water.
• Concentration: 0.1mg/ml after reconstitution.
• NCBI official symbol: CD40LG
• Accession #: P29965
• Protein GI: 231718
• NCBI gene ID#: 959
• NCBI official full name: CD40 ligand
• NCBI organism: Homo sapiens
• Peptide sequence: N/A
• SWISSPROT #: O75509
• Background Reference 1: N/A
• Background Reference 2: N/A
• Background Reference 3: N/A
• Background Reference 4: N/A
• Background Reference 5: N/A
• Source: CHO cells
• Species: Human
• By Source: CHO Cells
• By Species: Human
• Fusion tag: DDDDK Tag
• Sequence: Human CD40L (aa 116-261) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a DDDDK-tag.
• Biology activity: Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86) (ED50: <1ng/ml).
• Purity: >95% (SDS-PAGE).
• Endotoxin level is less than 0.01EU/ µg purified protein (LAL test
• Lonza).

Wysyłka i przechowywanie:

Stable for at least 6 months after receipt when stored at -20°C.

Inne:

Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.